Regular Production of Transgenic Wheat Mediated by Agrobacterium tumefaciens

Wheat is the number one crop both in acreage and total yield in the world. Therefore, it is very important to improve wheat by gene engineering techniques even though it belongs to the plants insensitive to gene transformation, especially to Agrobacterium-mediated transformation. Wheat immature embryos of 1.0-1.5mm in size, C58c1 of Agrobacterium strain harboring pPTN249, pPTN270, pPTN254, and pSIS-GFP respectively (all the vectors contain the aphA selectable gene driven by enhanced 35S promoter and a target gene controlled by ubi promoter or E35S promoter), AB medium for Agrobacterium activate culture, WCC medium for co-culture, were used in this study. The embryos with 4 days of pre-culture were transferred onto selection medium with 10mg/L geneticin, 50mg/L ticarcillin, 50mg/L vancomycin, and 50mg/L cefotaxine after 30 minutes of infection and 2 days of co-cultivation with Agrobacterium. Followed callus production,shoot regeneration on selection medium, 114 resistant plantlets were obtained from 10 transformation experiments of four genotypes. By nptⅡ ELISA (nptⅡ enzyme assay), PCR, Southern blot and leaf bleach,29 positive plants were identified from two genotypes of Bobwhite and Yanglmai 10, with an average transformation efficiency of 0.82 %. The result tested by Southern blot also showed that the transgenic plants with single- copy integration of target gene took 65.52% among total positive plants. The ELISA value of transgenic plant was also related to the copies of alien DNA integrating into wheat chromosomes, the transgenic plants with single copy integration giving higher ELISA value than the ones with 2 or 3 copies integration.     

加速我国现代农业一体化创新的路径与对策

农业现代化关键在于确立现代农业一体化创新支持路径。必须通过政府适度干预，克服现代农业一体化创新过程中的市场失灵，不断完善农业一体化创新的市场和政府激励机制，积极引导现代农业一体化创新主体的行为；通过政府诱导，实现现代农业创新的制度变迁，加快我国现代农业一体化创新步伐；通过深化农村金融改革，加速现代农业一体化创新，可以提高我国现代农业国际竞争的动态优势，促进“三农”问题解决，为实现农产品金融工具化、农业金融工程化奠定基础，实现现代农业与现代农村金融良性互动和谐发展。     

基于XML SCHEMA的异构生物信息集成研究

在后基因组时代,常有大规模的生物数据产生。这些生物数据的存贮形式各不相同,特别是与一些特定研究领域相关的数据,与其他数据的存贮形式差异较大。随着数据源的不同,数据模型的规范也不相同,同时,基于这些数据的应用服务各不相同。针对目前生物信息学中存在的异构生物数据现状,提出了一种基于XML Schema集成异构生物信息的方法。     

农业数字信息资源整合与共享

数字图书馆的建设是要拥有优质的数字信息资源，让每个人在任何时间、任何地点都能方便快捷地获取其所需要的知识资源，提高信息资源的利用效率。数字信息资源整合是期望利用先进的整合理论与思想，以及计算机技术、网络技术和数据库处理技术等先进的现代技术将数字化资源进行优化组合、聚类和重组，重新结合为一个新的有机整体，形成一个效能更好、效率更高的新的数字资源体系，高效率发挥各类资源的作用。     

"中国-东盟"经济一体化与广西新农业体系基本框架

文章结合"中国-东盟"经济一体化对广西农业发展的影响及广西的实际情况,阐述了广西构建新农业体系的必要性,提出在广西新农业体系中将水稻、玉米两大粮食作物种植业、桑蚕业列入战略产业和将水产、畜牧、甘蔗、水果、蔬菜、中药材、花卉等产业作为重点支柱产业的理论设想,以及促进战略产业和重点支柱产业发展的对策.     

网络教育与素质教育的整合

通过网络教育对素质教育影响的调查,探讨网络教育与素质教育整合的必然性,提出将网络教育与素质教育整合的教育理念。     

浸入式经历者框架——一种语言理解体验观

在体验认知思潮的影响下,ZWAAN(2004)提出了的浸入式经历者框架,认为理解者是所描述的情境的一位沉浸其中的经历者,理解就是对这一情境的身临其境的经历.浸入式经历者框架将语言加工划分为激活、解读和整合3个成份,与现实情境的事物、事件和事件系列对应,力图从体验的角度揭示语言理解的过程,为语言理解研究提供了一种思路.     

全域旅游背景下滁州市文旅融合高质量发展研究

全域旅游背景下的区域文旅有效融合及产业高质量发展，是新时代供给侧满足人们美好生活的重要改革措施，也是驱动区域产业结构优化和经济社会协调发展的有效途径。滁州市文旅资源较丰富，文旅产业发展速度较快，但产品的文化附加价值不高，文旅深度融合推进不够，整体形象不清晰。必须在基本理念、管理体系、资源场地、产品体系、空间载体等方面进行有机融合。深入挖掘地域文化的旅游资源潜质，创新文旅融合的产品呈现形式，打造高质量且独具特色的文旅精品项目，形成全域全新的文旅产业新业态，让文化旅游产业真正成为可持续发展的战略性支柱产业。     

高等学校与科研院所合并为我校图书馆全面发展提供了新的机遇

论述了西北农林科技大学图书馆抓住合并机遇，大胆进行改革。积极探索高等院校图书馆与科研院所图书馆合并的新模式。以“整合提高”为抓手，优化资源配置；实行科学化管理，规范化服务；根据学枝定位。进行科学规划；确立文献资源建设指导思想，形成特色馆藏体系；开展面向社会的信息服务。5年来．办馆条件实现了跨越式发展，图书馆数字化程度进一步提升，人员素质不断提高。为产学研结合、全面开创信息服务工作的新局面做出了贡献。     

Using global organic markets to pay for ecologically based agricultural development in China

The traditional command and control approach and the more recent free market have proven inadequate for promoting ecological agricultural development in China. Organic certification represents a regulated market mechanism with the potential to stimulate ecologically based agricultural research, extension, and investment. Recent linkages between the global organic food industry and local agricultural development in China provide an opportunity to test this potential. The article examines Chinas two largest organic certification systems for their potential to promote the adoption of integrated pest management (IPM) as a key component of ecological agriculture. Organic certification is providing a format for research, extension, and implementation of IPM principles and practices, and has the potential to do much more. However, possible contradictions between ecological and market rationality, inherent in organic certification and marketing systems, may be exacerbated by the authoritarian political economy of rural China.Paul Thiersis an assistant professor of Political Science at Washington State University Vancouver where he teaches comparative politics, political economy, and environmental policy in the Program in Public Affairs. His research focuses on the environmental, social, and political dynamics of globalization in rural China, with a particular emphasis on Chinas integration into global food trade and food standards.